tunel staining kit  (Servicebio Inc)

Journal: Chinese Herbal Medicines

Article Title: Isoquinoline alkaloids in Coptis chinensis to treat Alzheimer’s disease through promoting growth of Bifidobacterium breve inhibiting abnormal autophagy using a novel AI high-content intelligent imaging system

doi: 10.1016/j.chmed.2026.02.003

Figure Lengend Snippet: Co-administration of C. chinensis and B. breve S1 improved the pathological manifestations of AD mice. (A) Construction of B. breve S1 AD mice (* P < 0.05 ** P < 0.01 *** P < 0.001 vs D1, # P < 0.05 ## P < 0.01 ### P < 0.001 vs D7). (B) Statistical chart of latency time of Barnes maze test in AD mice ( n = 5). (C) Statistical chart of target quadrant ratio of Barnes maze test in mice ( n = 5). (D) Statistical chart of total displacement of Barnes maze test in mice ( n = 5). (E) The content of A β in serum ( n = 5). (F) The content of A β in brain ( n = 5). n. s., no significant difference, # P < 0.05 ### P < 0.001 vs Control group, * P < 0.05 ** P < 0.01 *** P < 0.001 vs Model-free group, n.s. not significant. (G) Results of Congo red staining (arrow: amyloid protein deposition, n = 5, scale bar: 500 μm, × 40). (H) Results of HE staining ( n = 5, scale bar: 50 μm, × 400). (I) Results of TUNEL staining (arrow: apoptotic cells, n = 5, scale bar: 50 μm, × 400).

Article Snippet: TUNEL staining kit (lot number: 01981015) was purchased from Shanghai Epizyme Biomedical Technology Co., Ltd. (Shanghai, China).

Techniques: Control, Staining, TUNEL Assay

Journal: Molecular Neurobiology

Article Title: Olfactory Mucosa Mesenchymal Stem Cell–Derived Exosomes Enhance Microglia M2 Polarization via the FGFR1/PLCγ1 Axis to Alleviate Alzheimer’s Disease

doi: 10.1007/s12035-026-05797-w

Figure Lengend Snippet: OM-MSCs-Exo alleviated cognitive impairment and neuroinflammation in AD mice through FGFR1. A Swimming distance, swimming time, number of platform arrivals, and latency to first entry ( n = 6). B The hippocampal tissues of mice were stained with HE. C Nissl staining was performed in the hippocampus of mice. D TUNEL assay. E Data plot of the TUNEL assay. F Levels of IL-1β, TNF-α, and IL-6 in mice hippocampus. G WB analysis of Aβ, p-Tau/Tau in mice hippocampus. H Aβ 1–42 levels were detected. I FGFR1 and PLCγ1 levels were measured. J Levels of p-NF-κB/NF-κB. K , L IF staining of CD86 and CD206 in mice hippocampus. M Levels of microglia M1 and M2 polarization–related factors in mice hippocampus ( n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001. Normality was confirmed using the Shapiro–Wilk test. Thereafter, data were analyzed with a one-way ANOVA (followed by Tukey’s post hoc test) for multiple-group comparisons

Article Snippet: According to the manufacturer’s instructions, sections were stained with a TdT-mediated dUTP nick-end labeling (TUNEL) staining kit (40306ES50; YEASEN) and a DAPI working solution under dark conditions.

Techniques: Staining, TUNEL Assay